STRC Web Prediction Jobs (2026-03-19)
Submitted Jobs
All three sequences submitted to each tool:
full_STRC(1775 aa) — positive controlIgK_SP_shorter_mini_STRC(1097 aa) — our constructshorter_mini_STRC_no_SP(1076 aa) — negative control
SignalP 6.0 — COMPLETE ✅
Job ID: 69BB889D0023BDA83618DAE9
URL: https://services.healthtech.dtu.dk/cgi-bin/webface2.cgi?jobid=69BB889D0023BDA83618DAE9
Results: see STRC Signal Peptide Validation
NetGPI 1.1 — COMPLETE ✅
Job ID: 69BB8C1F0023C6795E530357
URL: https://services.healthtech.dtu.dk/cgi-bin/webface2.cgi?jobid=69BB8C1F0023C6795E530357
| Construct | Prediction | Omega site | Likelihood |
|---|---|---|---|
| Full STRC | GPI-Anchored | S1749 | 0.471 |
| IgK + mini-STRC | GPI-Anchored | S1071* | 0.471 |
| No SP control | GPI-Anchored | S1050* | 0.471 |
All three: identical GPI signal. C-terminal is preserved. * = construct numbering.
DeepLoc 2.1 — COMPLETE ✅
Job ID: 69BB8C460023C6CDE1A692EF
URL: https://services.healthtech.dtu.dk/cgi-bin/webface2.cgi?jobid=69BB8C460023C6CDE1A692EF
| Construct | Top localization | Prob | Membrane type | Lipid anchor | SP |
|---|---|---|---|---|---|
| Full STRC | Extracellular | 74.1% | Soluble | 28.4% | Yes |
| IgK + mini-STRC | Extracellular | 57.4% | Lipid anchor | 72.1% | Yes |
| No SP control | Cell membrane | 42.9% | Lipid anchor | 71.4% | Artifact |
Key: mini-STRC lipid anchor 72.1% vs full STRC 28.4%. Truncation makes GPI signal more prominent.
NetNGlyc 1.0 — COMPLETE ✅
Job ID: 69BB95040023E1E80CE6DABF
Full STRC: 14 NxS/T sequons, 13 predicted glycosylated (threshold 0.5)
- Strongest: N65 NISS 0.757 (9/9), N476 NETL 0.707 (9/9), N565 NDTC 0.619 (9/9)
- Below threshold: N824 NDSV 0.452 (4/9)
- N696 NPSS in cut zone has PRO-X1 warning
IgK + mini-STRC: 5 sequons, ALL glycosylated ✅
| Position | Sequon | Score | Jury |
|---|---|---|---|
| N916 (=N238*) | NQSV | 0.707 | 8/9 |
| N964 (=N286*) | NGTL | 0.664 | 8/9 |
| N1179 (=N501*) | NLTL | 0.719 | 8/9 |
| N1274 (=N596*) | NESI | 0.567 | 6/9 |
| N824 (=N146*) | NDSV | 0.525 | 5/9 |
*construct numbering. SP doesn’t affect scores (within 0.003).
IUPred3 — COMPLETE ✅
UniProt: Q7RTU9 (Stereocilin, 1775 aa). Long disorder mode.
Total disordered: 110/1775 (6.2%)
| Region | Residues | % disordered | Avg score | AF3 pLDDT |
|---|---|---|---|---|
| N-terminal | 1-114 | 12.3% | 0.220 | 40.9 |
| Middle | 115-615 | 9.6% | 0.254 | 50.2 |
| Transition | 616-699 | 25.0% | 0.282 | 60.2 |
| Mini-STRC | 700-1775 | 2.5% | 0.187 | 71.2 |
Critical cross-validation at cut point:
- Res 691: IUPred3 0.819 (disordered) ↔ AF3 pLDDT 31.2 (lowest)
- Res 700: IUPred3 0.263 (ordered) ↔ AF3 pLDDT 57.1 (recovery)
- Two independent algorithms converge on same boundary
Disordered stretches: 73-86, 151-166, 200-208, 270-282, 332-339, 676-696 (cut zone), 1246-1248, 1459-1463, 1734-1752
FASTA Input File
/tmp/netgpi_input.fasta and /tmp/openclaw/uploads/netgpi_input.fasta
Connections
[supports]STRC Signal Peptide Validation — SignalP results[supports]STRC GPI-Anchor Analysis — NetGPI results[supports]STRC N-Glycosylation Analysis — NetNGlyc results[supports]STRC AF3 Truncation Boundary Sweep — IUPred3 cross-validates AF3 disorder[part-of]STRC Mini-STRC Single-Vector Hypothesis — computational validation suite[about]Misha