STRC N-Glycosylation Analysis
Key Finding
Truncation at residue 700 removes 9 of 14 N-glycosylation sites, but all 5 retained sites are confidently predicted functional by NetNGlyc 1.0. Average retained score (0.66) is comparable to full protein average (0.63).
Retained Sites (mini-STRC 700-1775)
| Full position | Sequon | Score | Jury | Notes |
|---|---|---|---|---|
| N824 | NDSV | 0.525 | 5/9 | Borderline but above threshold |
| N916 | NQSV | 0.707 | 8/9 | Strong |
| N964 | NGTL | 0.664 | 8/9 | Strong |
| N1179 | NLTL | 0.719 | 8/9 | Strongest retained site |
| N1274 | NESI | 0.567 | 6/9 | Moderate |
Lost Sites (N-terminal 1-699)
9 sites removed. Notable: N696 NPSS (0.577) sits in the disorder zone (IUPred3 0.82 at res 691) with a PRO-X1 warning, suggesting poor glycosylation efficiency even in full protein.
Implications
- Mini-STRC retains 36% of glycosylation sites (5/14)
- All retained sites are high-confidence (no marginal calls)
- IgK signal peptide doesn’t affect glycosylation prediction (scores within 0.003)
- Lost sites are mostly in disordered N-terminal (may not be efficiently glycosylated in vivo anyway)
- Whether 5 vs 14 glycans matters for STRC function is an experimental question
Connections
[supports]STRC Mini-STRC Single-Vector Hypothesis — glycosylation not a dealbreaker- STRC Web Prediction Jobs 2026-03-19 — NetNGlyc job results
[see-also]STRC GPI-Anchor Analysis — GPI anchor + glycosylation both post-translational[about]Misha