NetGPI
GPI-anchor prediction tool. Predicts whether a protein is GPI-anchored and the omega site (attachment point).
What It Does
- Predicts GPI-anchor signal presence
- Identifies omega site (where GPI is attached)
- Probability score for GPI modification
- Important for membrane-anchored proteins like stereocilin
How to Use
Web
- Go to https://services.healthtech.dtu.dk/services/NetGPI-1.1/
- Paste protein sequence
- View omega site prediction
Verified Status
VERIFIED — used for STRC GPI-anchor analysis. Confirmed GPI-anchor signal in STRC C-terminus.
STRC Research Usage
- STRC GPI-Anchor Analysis — confirmed GPI modification
- Critical for mini-STRC: must include GPI signal for stereocilia membrane attachment
- AF3 experiment showed mini-STRC (700-1780) with GPI signal at C-terminus → ipTM 0.91 (best TMEM145 binding)
- E1659A is near the GPI-anchor region (residue 1659 out of 1775) — variant could affect local folding near GPI attachment. AF3 shows structure intact (pTM 0.64)
Connections
- STRC GPI-Anchor Analysis [used-in]
- STRC Mini-STRC Single-Vector Hypothesis [see-also]
- SignalP [see-also] — N-terminal signal, NetGPI = C-terminal