DDGun — DDGun3D

Untrained algorithm predicting the folding stability impact of amino acid substitutions.

Key Info

• URL: https://github.com/biofold/ddgun
• Category: Protein Structure
• API: Yes
• Free: Free
• ACMG Relevance: PP3

Why It Matters

Entirely circumvents the overfitting pitfalls of supervised machine learning by relying exclusively on biophysical principles.

How to Use

See https://github.com/biofold/ddgun for documentation and access.

STRC Research Usage

PARTIAL — pip install succeeds but BioPython compatibility issues. Requires hhblits for full functionality. Untrained stability predictor would give unbiased ΔΔG.

Next Steps

• Test with STRC E1659A variant
• Verify API access
• Document specific results

Connections

• STRC Variant c.4976A>C — Misha [see-also]
• STRC Gene [about]