AAV Capsid Database
Database of adeno-associated virus (AAV) capsid variants and their tropism profiles. Essential for selecting the right AAV serotype for inner ear gene therapy.
What It Does
- Catalog of natural and engineered AAV capsids
- Tropism profiles (which tissues each serotype infects)
- Packaging capacity per serotype
- Neutralizing antibody cross-reactivity data
- Engineering strategies (directed evolution, rational design)
How to Use
Web
- https://aavdb.org (if available) or search literature
- Also see: AAV Atlas project
Key Serotypes for Inner Ear
| Serotype | Inner Ear Tropism | Notes |
|---|---|---|
| AAV1 | IHC + OHC | Good transduction, used in OTOF trials |
| AAV2 | IHC primarily | Historical default |
| AAV9 | Broad CNS + some IHC | BBB crossing |
| AAV-PHP.eB | CNS | May reach spiral ganglion |
| Anc80L65 | OHC + IHC | Ancestral reconstructed, good for cochlea |
| AAV-ie | IHC + OHC | Engineered for inner ear |
Verified Status
VERIFIED — compiled inner ear AAV serotype data from literature:
- Anc80L65: IHC + OHC tropism, 5-10% seroprevalence, BEST candidate for STRC (Landegger 2017)
- AAV1: IHC + OHC, ~30% seroprevalence, used in OTOF trials
- AAV9: IHC + some OHC, ~40% seroprevalence
- AAV-ie: IHC + OHC engineered, seroprevalence unknown (Tan 2019)
- STRC CDS 5,325bp exceeds AAV limit (~4.7kb) → mini-STRC (700-1775) = 3,228bp FITS all serotypes
STRC Research Usage
- STRC AAV Vector Design — serotype selection for STRC delivery
- STRC Gene Therapy Landscape 2026 — vector design context
- STRC is 5,427 bp CDS — exceeds AAV packaging limit (~4.7kb) → needs mini-STRC or dual-vector approach
Results (April 2026)
- Capacity analysis DONE: mini-STRC (700-1775) = 3,228bp → FITS all AAV serotypes (limit ~4.7kb). Full STRC 5,325bp does NOT fit.
- OHC serotypes DONE: Anc80L65 and AAV-ie have best OHC tropism. AAV1 also transduces OHC.
- Immune profile DONE: Anc80L65 ~5-10% seroprevalence (lowest). AAV1 ~30%, AAV9 ~40%. Misha age 4 = low seroprevalence window.
- Next: delivery route comparison (round window vs canalostomy) — requires clinical literature review
Connections
- STRC AAV Vector Design [used-in]
- STRC Gene Therapy Landscape 2026 [used-in]
- STRC Mini-STRC Single-Vector Hypothesis [see-also]
- STRC Dual-Vector vs Single-Vector Transduction [see-also]
- STRC Anti-AAV Immune Response Model [see-also]