h05 log

2026-04-24

Ranking delta A → C. Post-batch-2 review: 6 load-bearing parameters (STRC mRNA t½, STRC protein t½, STRC copy #/OHC, CRE K_half, PDE4 Vmax OHC, CaMKII k_auto) confirmed without primary literature — 3 of them STRC-specific with no measurement anywhere (Sharma 2018 / Krey 2015 retracted as phantom). Model cannot run quantitatively; A-tier unjustifiable. next_step rewritten to wet-lab + lit-absence documentation. Trigger: vault-wide audit review post 2026-04-25 batch — Lit Audit Status 2026-04-25.

Second lit-audit pass complete: 4 additional phantoms resolved (CaMKII PMID 21458670→21884935, Mammano 1999 Pflugers→Yamoah 1998+Beurg 2010, Müller 2002→Hackney 2005, Houslay 2010→Owens 1997). 7 paper notes created. STRC mRNA t½, protein t½, copy number confirmed NOT MEASURED in primary literature; scripts updated. lit_audit→partial. A held. → STRC h05 Parameter Provenance Audit 2026-04-25

Misha-specific PD: realistic affinity penalty 10-100× outside feasibility window; acoustic therapy = free adjunct → STRC Ca Oscillation Maternal-Only PD
No tier change; stays A. Next: SPR/BLI E1659A affinity penalty measurement.
Parameter provenance audit complete + post-audit fix applied: 4 phantom cites removed (Wu 2011, Sharma 2018, Cha 2010, Krey 2015), Chao 2010→Chao 2011 (PMID 21458670), PKA K_cAMP cite corrected to Surdo 2017 (PMID 29074866). CREB-P dephos 2-4× faster than lit flagged, STRC mRNA/protein t½ cross-script mismatches (4× and 20×) flagged explicitly. Phase 3 topological verdict defensible. Tier A held. → STRC h05 Parameter Provenance Audit 2026-04-23

Phase 3 globally stable; max Re(λ) = −5×10⁻⁶/s; true Hill n=4.33 confirmed → STRC Calcium Oscillation Acoustic Therapy
Phase 2 robustness index 0.91; Phase 1 RBM24 ODE baseline established → STRC Calcium Oscillation Acoustic Therapy
Initial assignment: A-tier, Mech 3, Deliv 4, Misha 2 → STRC Hypothesis Ranking

#5 Ca oscillation: stays A. Maternal-allele-only PD: rescue only if penalty ≤3× at fold ≥1.3. Most plausible 10-100×. → STRC Ca Oscillation Maternal-Only PD
#5 Ca Oscillation post-audit fix: A held. 4 phantoms removed (Wu 2011, Sharma 2018, Cha 2010, Krey 2015). PKA K_cAMP cite Zaccolo 2007→Surdo 2017 (PMID 29074866); Chao 2010→Chao 2011 (PMID 21458670). CREB dephos 2-4× faster than lit flagged. STRC mRNA/protein t½ cross-script 4× and 20× inconsistencies flagged. Phase 3 topological verdict robust. → STRC h05 Parameter Provenance Audit 2026-04-23